Nitrogen deposition and soil microbiology

Summary

This project made an inventory of the soil microbial communities in the organic layer of the D (n=6) and E (n=6) plots at the Svartberget nitrogen addition experiment in 2016, after 20 years of annual N addition at 12.5 and 50 kg N ha-1 yr-1. We used PLFA profiling and fungal DNA sequencing to study shifts in the microbial community structure, and used enzyme assays to study the impact of nitrogen on the soil metabolism targeting key components of soil organic matter and litter, including cellulose and hemicellulose, organic nitrogen in peptides and microbial necromass, phosphates, and lignin.

fungal communities nitrogen deposition soil microbiology